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Figure 2 | BMC Genomics

Figure 2

From: A Bead-based Normalization for Uniform Sequencing depth (BeNUS) protocol for multi-samples sequencing exemplified by HLA-B

Figure 2

Percentage of read numbers among 96 multiplexing indexes. The horizontal axis shows the 96 samples and the vertical axis shows read percentage among demultiplexed 96 FASTQ files. Red bars show distribution of read number in proportion to libraries prepared by BioAnalyzer and agarose gel size selection, and blue bars show result by BeNUS method. Sequence reads for each sample were counted for evaluation of the normalization step in library preparation.

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