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Table 1 Alignment of the HLA-B sequence and genotype in 96 samples

From: A Bead-based Normalization for Uniform Sequencing depth (BeNUS) protocol for multi-samples sequencing exemplified by HLA-B

Sample

HLA-B SSO

HLA-B PSP*

Depth

Note

Allele 1

Allele 2

Allele 1

Allele 2

Allele 1

Allele 2

0554

HLA-B*44:03

HLA-B*40:06

HLA-B*44:03:01:01

HLA-B*40:06:01:01

1543.4

1114.5

 

0555

HLA-B*40:01

HLA-B*44:03

HLA-B*40:01:02

HLA-B*44:03:01

2228.5

2084.8

 

0556

HLA-B*13:01

HLA-B*46:01

HLA-B*13:01:01

HLA-B*46:01:01

860.5

765

 

0560

HLA-B*13:01

HLA-B*51:01

HLA-B*13:01:01

HLA-B*51:01:01

1416.5

1455.9

 

0605

HLA-B*52:01

HLA-B*52:01

HLA-B*52:01:01:02

HLA-B*52:01:01:02

6928.7

Homozygous

0607

HLA-B*07:02

HLA-B*07:02

HLA-B*07:02:01

HLA-B*07:02:01

7357.3

Homozygous

0616

HLA-B*40:02

HLA-B*52:01

HLA-B*40:02:01

HLA-B*52:01:01:02

513.2

500.6

 

0639

HLA-B*40:06

HLA-B*35:01

HLA-B*40:06:01:01

HLA-B*35:01:01:02

1758

1656.8

 

0642

HLA-B*40:01

HLA-B*35:01

HLA-B*40:01:02

HLA-B*35:01:01:02

2016.8

1545.3

 

0645

HLA-B*54:01

HLA-B*54:01

HLA-B*54:01:01

HLA-B*54:01:01

6537

Homozygous

0649

HLA-B*55: 02

HLA-B*40:02

HLA-B*55:02:01

HLA-B*40:02:01

1402.3

1635.5

 

0652

HLA-B*15:01

HLA-B*35:01

HLA-B*15:01:01:01

HLA-B*35:01:01:02

2395.4

2308.4

 

0658

HLA-B*56:01

HLA-B*44:03

HLA-B*56:01:01

HLA-B*44:03:01

1686.7

2074

 

0663

HLA-B*13:01

HLA-B*52:01

HLA-B*13:01:01

HLA-B*52:01:01:02

1538.1

1455.7

 

0666

HLA-B*54:01

HLA-B*38:02

HLA-B*54:01:01

HLA-B*38:02:01

2105

1790.8

 

0703

HLA-B*55:02

HLA-B*40:06

HLA-B*55:02:01

HLA-B*40:06:01:01

1173.1

1445.3

 

0735

HLA-B*44:03

HLA-B*38:02

HLA-B*44:03:01

HLA-B*38:02:01

2507.5

1998.5

 

0739

HLA-B*40:02

HLA-B*48:01

HLA-B*40:02:01

HLA-B*48:01:01

1947.1

2382.9

 

0741

HLA-B*59:01

HLA-B*07:02

HLA-B*59:01:01:01

HLA-B*07:02:01

1458.6

2336

 

0772

HLA-B*07:02

HLA-B*48:01

HLA-B*07:02:01

HLA-B*48:01:01

3512.9

3425

 

0779

HLA-B*44:02

HLA-B*39:01

HLA-B*44:02:01:01

HLA-B*39:01:01:03

1677.5

1060.6

 

0784

HLA-B*40:06

HLA-B*15:01

HLA-B*40:06:01:01

HLA-B*15:01:01:01

1875.1

1820.8

 

0785

HLA-B*52:01

HLA-B*51:01

HLA-B*52:01:01:01

HLA-B*51:01:01

6041.4

6018.4

Onlu 4 heterozygous SNVs

0810

HLA-B*40:02

HLA-B*46:01

HLA-B*40:02:01

HLA-B*46:01:01

560.8

546.8

 

0815

HLA-B*35:01

HLA-B*07:02

HLA-B*35:01:01

HLA-B*07:02:01

1239.5

1740

 

0821

HLA-B*40:01

HLA-B*40:02

HLA-B*40:01:02

HLA-B*07:02:01

1239.5

1740

 

0821

HLA-B*40:01

HLA-B*40:02

HLA-B*40:01:02

HLA-B*40:02:01

2144.4

1925.5

 

0822

HLA-B*54:01

HLA-B*35:01

HLA-B*54:01:01

HLA-B*35:01:01

1550

1840.6

 

0823

HLA-B*15:11

HLA-B*07:02

HLA-B*15:11:01

HLA-B*07:02:01

703.5

914.6

 

0830

HLA-B*51:01

HLA-B*48:01

HLA-B*51:01:01

HLA-B*48:01:01

1833.8

2509

 

0969

HLA-B*40:06

HLA-B*51:01

HLA-B*40:06:01:01

HLA-B*51:01:01

1575.4

1650.4

 

0975

HLA-B*44:03

HLA-B*07:02

HLA-B*44:03:01

HLA-B*07:02:01

180.4

209.3

 

0976

HLA-B*54:01

HLA-B*35:01

HLA-B*54:01:01

HLA-B*35:01:01

3635.9

3759.2

 

0977

HLA-B*44:02

HLA-B*15:18

HLA-B*44:02:01:01

HLA-B*15:18:01

722.2

574.4

 

0979

HLA-B*39:01

HLA-B*39:01

HLA-B*39:01:01:03

HLA-B*39:01:03

2128

2125.7

Only 1 heterozygous SNV

0991

HLA-B*15:01

HLA-B*39:01

HLA-B*15:01:01

HLA-B*39:01:01:03

1685.4

1551.3

 

0997

HLA-B*15:01

HLA-B*51:01

HLA-B*15:01:01:01

HLA-B*39:01:01:03

1685.4

1551.3

 

0997

HLA-B*15:01

HLA-B*51:01

HLA-B*15:01:01

HLA-B*51:01:01

2588

2692.1

 

0999

HLA-B*48:01

HLA-B*39:01

HLA-B*48:01:01

HLA-B*39:01:01:03

1470.4

1045.3

 

1009

HLA-B*54:01

HLA-B*39:01

HLA-B*54:01:01

HLA-B*39:01:01:03

326.8

294.7

 

1011

HLA-B*52:01

HLA-B*07:02

HLA-B*52:01:01:02

HLA-B*07:02:01

730.9

943.7

 

1012

HLA-B*35:01

HLA-B*48:01

HLA-B*35:01:01:02

HLA-B*48:01:01

1830.1

2482.1

 

1013

HLA-B*54:01

HLA-B*52:01

HLA-B*54:01:01

HLA-B*52:01:01:02

533.9

600.7

 

1014

HLA-B*55:02

HLA-B*51:01

HLA-B*55:02:01

HLA-B*51:01:01

970.1

1153.1

 

1016

HLA-B*44:03

HLA-B*35:01

HLA-B*44:03:01

HLA-B*35:01:01

739.7

655.5

 

1018

HLA-B*46:01

HLA-B*15:01

HLA-B*46:01:01

HLA-B*15:01:01:01

5360.8

5496.1

Only 7 heterozygous SNVs

1030

HLA-B*40:06

HLA-B*07:02

HLA-B*40:06:01:01

HLA-B*07:02:01

1638.8

2668.6

 

1045

HLA-B*15:11

HLA-B*35:01

HLA-B*15:11:01

HLA-B*35:01:01:02

1597.5

1448.8

 

1056

HLA-B*58:01

HLA-B*67:01

HLA-B*58:01:01

HLA-B*67:01:01

2497.9

2214.5

 

1057

HLA-B*40:02

HLA-B*40:02

HLA-B*40:02:01

HLA-B*40:02:01

4113.6

Homozygous

1064

HLA-B*40:06

HLA-B*51:01

HLA-B*40:06:01:01

HLA-B*51:01:01

963.5

1048.8

 

1065

HLA-B*54:01

HLA-B*52:01

HLA-B*54:01:01

HLA-B*52:01:01:02

1213.9

1563.8

 

1071

HLA-B*56:01

HLA-B*40:06

HLA-B*56:01:01

HLA-B*40:06:01:01

4061.2

1223

 

1079

HLA-B*40:02

HLA-B*39:01

HLA-B*40:02:01

HLA-B*39:01:03

2319.8

1507.5

 

1082

HLA-B*40:01

HLA-B*51:01

HLA-B*40:01:02

HLA-B*51:01:01

639.3

542.7

 

1083

HLA-B*59:01

HLA-B*54:01

HLA-B*59:01:01

HLA-B*54:01:01

716.5

1208.2

 

1087

HLA-B*44:03

HLA-B*07:02

HLA-B*44:03:01

HLA-B*07;02:01

1975.5

2605.1

 

1095

HLA-B*40:02

HLA-B*54:01

HLA-B*40:02:01

HLA-B*51:01:01

946.4

962.2

 

1097

HLA-B*40:01

HLA-B*40:06

HLA-B*40:01:02

HLA-B*40:06:01:01

2642.2

1822.5

 

1110

HLA-B*40:01

HLA-B*51:01

HLA-B*40:01:02

HLA-B*51:01:01

1277.1

1156.9

 

1111

HLA-B*54:01

HLA-B*40:03

HLA-B*54:01:01

HLA-B*40:03

831.3

943.7

 

1116

HLA-B*52:01

HLA-B*07:02

HLA-B*52:01:01:02

HLA-B*07:02:01

981.1

1535.6

 

1117

HLA-B*52:01

HLA-B*51:01

HLA-B*52:01:01:02

HLA-B*51:01:01

5900.6

6084.9

Only 3 heterozygous SNVs

1119

HLA-B*35:01

HLA-B*07:02

HLA-B*35:01:01:02

HLA-B*07:02:01

921.3

1370.1

 

1129

HLA-B*59:01

HLA-B*40:02

HLA-B*59:01:01:01

HLA-B*40:02:01

1472.9

2178.5

 

1131

HLA-B*44:03

HLA-B*52:01

HLA-B*44:03:01

HLA-B*52:01:01:02

1519.9

1456.8

 

1142

HLA-B*40:02

HLA-B*39:04

HLA-B*40:02:01

HLA-B*39:04

1253

886.5

 

1144

HLA-B*44:03

HLA-B*15:11

HLA-B*44:03:01

HLA-B*15:11:01

1061.7

993.2

 

1149

HLA-B*44:03

HLA-B*52:01

HLA-B*44:03:01

HLA-B*52:01:01:02

719.6

633.6

 

1154

HLA-B*59:01

HLA-B*40:02

HLA-B*59;01:01:01

HLA-B*40:02:01

146.2

171.8

 

1157

HLA-B*35:01

HLA-B*48:01

HLA-B*35:01:01:02

HLA-B*48:01:01

911

1077.3

 

1160

HLA-B*59:01

HLA-B*54:01

HLA-B*59:01:01:02

HLA-B*54:01:01

129.8

166.8

 

1161

HLA-B*55:02

HLA-B*51:01

HLA-B*55:02:01

HLA-B*51:01:01

402.5

366.6

 

1163

HLA-B*56:01

HLA-B*46:01

HLA-B*56:01:01

HLA-B*46:01:01

2048.1

2276.5

 

1165

HLA-B*15:01

HLA-B*07:02

HLA-B*15:01:01:01

HLA-B*07:02:01

688.1

907.3

 

1167

HLA-B*40:06

HLA-B*52:01

HLA-B*40:06:01:01

HLA-B*52:01:01:02

1486.7

4508.1

 

1169

HLA-B*54:01

HLA-B*54:01

HLA-B*54:01:01

HLA-B*54:01:01

7086.1

Homozygous

1171

HLA-B*54:01

HLA-B*44:03

HLA-B*54:01:01

HLA-B*44:03:01

1586.6

1833.9

 

1175

HLA-B*15:11

HLA-B*15:01

HLA-B*15:11:01

HLA-B*HLA-B*15:01::01:01

5717.6

5693

Only 3 heterozygous SNVs

1182

HLA-B*55:02

HLA-B*51:01

HLA-B*55:02:01

HLA-B*51:01:01

1156.6

1451.5

 

1183

HLA-B*54:01

HLA-B*35:01

HLA-B*54:01:01

HLA-B*35:01:01

1413.5

1537.7

 

1184

HLA-B*52:01

HLA-B*52:01

HLA-B*52:01:01:02

HLA-B*52:01:01:02

6701.3

Homozygous

1199

HLA-B*51:01

HLA-B*51:01

HLA-B*51:01:01

HLA-B*51:01:01

5229.1

Homozygous

1201

HLA-B*44:03

HLA-B*52:01

HLA-B*44:03:01

HLA-B*52:01:01:02

1990.6

1665.9

 

1203

HLA-B*54:01

HLA-B*52:01

HLA-B*54:01:01

HLA-B*52:01:01:02

1246.4

1313.2

 

1217

HLA-B*40:01

HLA-B*46:01

HLA-B*40:01:02

HLA-B*46:01:01

2225.1

1882.2

 

1224

HLA-B*40:02

HLA-B*40:03

HLA-B*40:02:01

HLA-B*40:03

6306.5

6286.4

Only 3 heterozygous SNVs

1225

HLA-B*40:01

HLA-B*44:03

HLA-B*40:01:02

HLA-B*44:03:01

1554

1399.6

 

1229

HLA-B*52:01

HLA-B*52:01

HLA-B*52:01:01:02

HLA-B*52:01:01:02

6748.5

Homozygous

1234

HLA-B*44:03

HLA-B*40:06

HLA-B*44:03:01

HLA-B*40:06:01:01

2490.3

1715.9

 

1236

HLA-B*54:01

HLA-B*40:06

HLA-B*54:01:01

HLA-B*40:06:01:01

1132.4

1244.4

 

1238

HLA-B*40:02

HLA-B*67:01

HLA-B*40:02:01

HLA-B*67:01:01

2040.9

1691

 

1239

HLA-B*40:03

HLA-B*51:01

HLA-B*40:03

HLA-B*51:01:01

1787

1789.8

 

1250

HLA-B*44:03

HLA-B*51:01

HLA-B*44:03:01

HLA-B*51:01:01

357.7

317.8

 

1259

HLA-B*40:02

HLA-B*07:02

HLA-B*40:02:01

HLA-B*07:02:01

1346.9

1934.4

 

1260

HLA-B*40:06

HLA-B*35:01

HLA-B*40:06:01:01

HLA-B*35:01:01:02

641.9

680.8

 

1265

HLA-B*40:01

HLA-B*40:06

HLA-B*40:01:02

HLA-B*40:06:01:01

2226.2

1691.7

 
  1. *Phase defined sequencing pipeline.