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Table 6 Robustness of the gene stability determination method

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

  Full dataset ranking comparison Comparative delta-Ct Bestkeeper (SD) NormFinder GeNorm
3 samples per experimental group subsets Identical ranking 4 11 1 8
  Top 3 genes in different order 13 11 21 1
  Different ranking 8 3 2 16
2 samples per experimental group subsets identical ranking 2 11 4 4
  Top 3 genes in different order 11 9 18 4
  Different ranking 12 5 3 17
Total Identical ranking 12% 44% 10% 24%
  Top 3 genes in different order 48% 40% 78% 10%
  Different ranking 60% 16% 12% 66%
  1. Similarity of 50 subsets stability rankings by each method and the ranking obtained with the whole dataset. 25 subsets are formed by 3 samples per group (age/temperature) and another 25 subsets have 2 samples per group.