Table 6 Robustness of the gene stability determination method
Full dataset ranking comparison | Comparative delta-Ct | Bestkeeper (SD) | NormFinder | GeNorm | |
|---|---|---|---|---|---|
3 samples per experimental group subsets | Identical ranking | 4 | 11 | 1 | 8 |
Top 3 genes in different order | 13 | 11 | 21 | 1 | |
Different ranking | 8 | 3 | 2 | 16 | |
2 samples per experimental group subsets | identical ranking | 2 | 11 | 4 | 4 |
Top 3 genes in different order | 11 | 9 | 18 | 4 | |
Different ranking | 12 | 5 | 3 | 17 | |
Total | Identical ranking | 12% | 44% | 10% | 24% |
Top 3 genes in different order | 48% | 40% | 78% | 10% | |
Different ranking | 60% | 16% | 12% | 66% |