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Table 9 Efficiency-corrected delta Cqs by temperature group with each efficiency + reference gene combination

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

  High T Cq Mean High T Cq SD Normal T Cq Mean Normal T Cq SD Low T Cq Mean Low T Cq SD
CYP19a UBQ + RPS4 LinRegPCR 1.11 4.33 -0.35 3.54 -0.74 5.41
CYP19a UBQ + RPS4 PCR-Miner 1.19 4.65 -0.38 3.81 -0.8 5.81
CYP19a B2M LinRegPCR 0.78 5.26 -0.89 4 0.24 5.8
CYP19a B2M PCR-Miner 0.83 5.66 -0.96 4.3 0.26 6.24
AMH UBQ + RPS4 LinRegPCR 0.07 3.39 1.02 4.47 -1.27 1.48
AMH UBQ + RPS4 PCR-Miner 0.07 3.65 1.09 4.8 -1.36 1.57
AMH B2M LinRegPCR -0.26 3.67 0.5 4.23 -0.32 2.16
AMH B2M PCR-Miner -0.28 3.94 0.54 4.54 -0.34 2.32
SOX19 UBQ + RPS4 LinRegPCR -0.24 3.99 -0.92 3.83 1.33 2.39
SOX19 UBQ + RPS4 PCR-Miner -0.26 4.28 -0.98 4.11 1.42 2.56
SOX19 B2M LinRegPCR -0.57 5.19 -1.46 3.97 2.31 3.08
SOX19 B2M PCR-Miner -0.62 5.57 -1.56 4.26 2.48 3.31
SOX9 UBQ + RPS4 LinRegPCR 0.38 1.82 0.17 1.44 -0.59 1.13
SOX9 UBQ + RPS4 PCR-Miner 0.4 1.96 0.18 1.55 -0.64 1.21
SOX9 B2M LinRegPCR 0.04 2 -0.37 1.75 0.39 2.05
SOX9 B2M PCR-Miner 0.05 2.15 -0.4 1.88 0.42 2.21
SOX17 UBQ + RPS4 LinRegPCR 0.58 2.12 -0.6 1.54 0.09 1.59
SOX17 UBQ + RPS4 PCR-Miner 0.62 2.26 -0.63 1.65 0.09 1.7
SOX17 B2M LinRegPCR 0.25 3.47 -1.13 2.06 1.07 2.39
SOX17 B2M PCR-Miner 0.26 3.72 -1.21 2.21 1.15 2.56
VASA UBQ + RPS4 LinRegPCR 1.26 1.46 -0.96 4.04 -0.19 2.6
VASA UBQ + RPS4 PCR-Miner 1.35 1.57 -1.03 4.34 -0.21 2.79
VASA B2M LinRegPCR 0.93 1.91 -1.5 3.8 0.79 2.46
VASA B2M PCR-Miner 0.99 2.05 -1.61 4.08 0.85 2.64
  1. Mean efficiency-corrected delta Cqs and SD values for the three rearing temperatures (T): high, normal and low; in the four datasets produced after efficiency correction with LinRegPCR or PCR-Miner and later normalization with UBQ + RPS4 or B2M.