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Table 9 Efficiency-corrected delta Cqs by temperature group with each efficiency + reference gene combination

From: Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

 

High T Cq Mean

High T Cq SD

Normal T Cq Mean

Normal T Cq SD

Low T Cq Mean

Low T Cq SD

CYP19a UBQ + RPS4 LinRegPCR

1.11

4.33

-0.35

3.54

-0.74

5.41

CYP19a UBQ + RPS4 PCR-Miner

1.19

4.65

-0.38

3.81

-0.8

5.81

CYP19a B2M LinRegPCR

0.78

5.26

-0.89

4

0.24

5.8

CYP19a B2M PCR-Miner

0.83

5.66

-0.96

4.3

0.26

6.24

AMH UBQ + RPS4 LinRegPCR

0.07

3.39

1.02

4.47

-1.27

1.48

AMH UBQ + RPS4 PCR-Miner

0.07

3.65

1.09

4.8

-1.36

1.57

AMH B2M LinRegPCR

-0.26

3.67

0.5

4.23

-0.32

2.16

AMH B2M PCR-Miner

-0.28

3.94

0.54

4.54

-0.34

2.32

SOX19 UBQ + RPS4 LinRegPCR

-0.24

3.99

-0.92

3.83

1.33

2.39

SOX19 UBQ + RPS4 PCR-Miner

-0.26

4.28

-0.98

4.11

1.42

2.56

SOX19 B2M LinRegPCR

-0.57

5.19

-1.46

3.97

2.31

3.08

SOX19 B2M PCR-Miner

-0.62

5.57

-1.56

4.26

2.48

3.31

SOX9 UBQ + RPS4 LinRegPCR

0.38

1.82

0.17

1.44

-0.59

1.13

SOX9 UBQ + RPS4 PCR-Miner

0.4

1.96

0.18

1.55

-0.64

1.21

SOX9 B2M LinRegPCR

0.04

2

-0.37

1.75

0.39

2.05

SOX9 B2M PCR-Miner

0.05

2.15

-0.4

1.88

0.42

2.21

SOX17 UBQ + RPS4 LinRegPCR

0.58

2.12

-0.6

1.54

0.09

1.59

SOX17 UBQ + RPS4 PCR-Miner

0.62

2.26

-0.63

1.65

0.09

1.7

SOX17 B2M LinRegPCR

0.25

3.47

-1.13

2.06

1.07

2.39

SOX17 B2M PCR-Miner

0.26

3.72

-1.21

2.21

1.15

2.56

VASA UBQ + RPS4 LinRegPCR

1.26

1.46

-0.96

4.04

-0.19

2.6

VASA UBQ + RPS4 PCR-Miner

1.35

1.57

-1.03

4.34

-0.21

2.79

VASA B2M LinRegPCR

0.93

1.91

-1.5

3.8

0.79

2.46

VASA B2M PCR-Miner

0.99

2.05

-1.61

4.08

0.85

2.64

  1. Mean efficiency-corrected delta Cqs and SD values for the three rearing temperatures (T): high, normal and low; in the four datasets produced after efficiency correction with LinRegPCR or PCR-Miner and later normalization with UBQ + RPS4 or B2M.