Figure 6

GR binds genomic sites associated with genes encoding Dex-induced TFs. (A) ChIP-seq data from BMMФ (black; see also Additional file 3: Figure S4), adipocytes (crosshatched) and C2C12 cells (red) reveal multiple Dex-dependent GR binding peaks associated with Dex-induced genes encoding TFs. The numbers under the putative GR binding sites indicate primer pairs used in ChIP-qPCR experiments in BMMФ (Additional file 2: Table S3). (B) ChIP-qPCR-assessed GR occupancy at putative GR binding sites indicated in (A) prior (U) and upon (D) a 40-min stimulation with 100 nM Dex. (C) Scanning ChIP of the Klf4 genomic region reveals a putative GR binding site. Primer positions and amplified products are shown as black rectangles over the gene schematics. Putative sites harboring sequences similar to GR binding sites are labeled as GRE1,2,3 with the binding sequence shown on top. The capital letters indicate nucleotides fitting a consensus GRE. A green rectangle overlapping the Klf4 TSS indicates the position of a KLF4 binding site identified in [36]. Data for each site represent average of 2 or more independent experiments; error bars are standard deviation.