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Table 1 Mapping statistics

From: Influence of RNA extraction methods and library selection schemes on RNA-seq data

Group Sample All reads Mapped reads Pairs Mapped, MAPQ > 1 Mapped on exons (%) Mapped on introns (%) Mapped on introns (%) mapped to non annotated regions (%) Mapped on rRNA (%) Mapped on mitochondrial DNA (%) Unique starting position (%)
PolyA Trizol Tot_RNA1 101028616 93699257 78996022 83038355 86,7 10,7 2,6 0,31 4,80 32,4
Tot_RNA2 103480788 96072826 81214720 84983881
PolyA Qiagen Tot_RNA3 100766522 100766522 76780490 82231228 91,3 6,6 2,1 0,30 4,58 28,5
Tot_RNA4 109645220 109645220 83720316 89300384
RiboZ Trizol Tot_RNA1 109221096 103587793 92825224 85242138 60,6 35,1 4,3 0,04 0,39 51,5
Tot_RNA2 113936740 107997806 96763362 90980604
RiboZ Qiagen Tot_RNA3 118950948 118950948 110394536 91193892 81,6 15,6 2,8 0,02 0,28 35,1
Tot_RNA4 112056778 112056778 88450950 86755352
RiboZ cytoplasmic Cyt_RNA5 111517838 102989353 86770814 87248867 87,1 10,3 2,6 0,03 0,56 31,3
Cyt_RNA6 102998772 94630661 79109400 87248867
RiboZ nuclear Nuc_RNA7 126297526 122854406 116663678 115579529 31,1 60,8 8,2 0,01 0,05 65,2
Nuc_RNA8 127905406 124011269 117109650 116428981
  1. The numbers of mapped sequence reads are given as absolute numbers. Percentages are calculated according to the total number of reliable reads (MAPQ > 1). The number of unique starting positions is a measure of the library complexity.