Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine

Table 1 Genome statistics

Feature	“Ca. M. alvus”	“Ca. M. intestinalis”	M. luminyensis
Genome size^a	1,666,795	1,931,651	2,637,810^d
Genome size^a	1,666,795	1,931,651	(2,620,233)
DNA G + C content	55.6%	41.3%	60.5%
% DNA coding region	89.5%	88.4%	87.6%
Intergenic regions mean size (SD)^a	102 (175)	119 (264)	121 (238)
Genes mean G + C content	56.3%	42.4%	61.0%
Putative replicons	1(+1)^b	1(+1)^b	1 (+1)^b
Extrachromosomal elements	NA^c	NA^c	NA^c
Total genes	1,705	1,882	2,713
RNA genes	52	50	52
rRNA genes (5S-16S-23S)	4 (2 - 1 - 1)	4 (2 - 1 - 1)	4 (2 - 1 - 1)
tRNA genes	48	46	48
Protein coding genes	1,653	1,832	2,661
Mean size of protein coding genes (SD)^a	901 (667)	930 (890)	859 (676)
Median size of protein coding genes^a	771	780	732
Gene products with function prediction	1,335	1,476	2,002
Gene products assigned to arCOGs	1,271	1,438	2,065
Gene products assigned Pfam domains	123	125	204
Gene products with signal peptides	247	336	512
Gene products with transmembrane helices	281	389	585
CRISPR repeats	1^e	1	1

^aSizes are given in bp.
^bPresence of two different cdc6 genes per genome. See the text for more information.
^cNot available.
^dData from [8]: in bracket stands the total bp (26 contigs) available from database [GenBank: CAJE01000001 to CAJE01000026], analyzed in this study.
^ePresence of CRISPR repeats split into two neighboring loci (see Additional file 1: Table S3) surrounding a DNA sequence containing one gene encoding a putative transposase.

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