Figure 5

Transcription of TK1361 (MCM2) and TK1620 (MCM3). (A) As annotated in the T. kodakarensis genome [38], TK1361 has an atypical 5'-extension, here shown by broken lines. The location of the TSS identified for TK1361 and the sequence downstream that could function as a translation start site are shown. With the TSS re-categorized from iTSS to pTSS and translation initiated at the boxed GTG codon, the MCM2 generated has a standard MCM structure. (B) The organization of the TK1619-TK1620 (MCM3) region. The locations and upstream promoter sequences for the pTSS and an iTSS within TK1620 are indicated. A putative GTG translation initiation codon downstream of the iTSS is boxed. In both panels the protein domains, identified in the NCBI Conserved Domain Database [58], are shown in dark grey. The abundances of transcripts present in T. kodakarensis cells growing exponentially (S_exp; blue) and in stationary phase (S_stat; red) in sulfur medium, growing exponentially in pyruvate medium before (P_exp; green) and 20 min after sulfur addition (PS; orange) are given by the peak heights. Data from the control library (C) not digested with TEX are shown in grey. The relative abundance scales on the right of each panel allow direct comparisons of all data in that panel. The black scale bar in the top right corner of each panel is corresponds to 100 nt.