Discovery of common sequences absent in the human reference genome using pooled samples from next generation sequencing

Table 2 Frequency of micSeqs from validation samples

micSeqID	Freq from validation data of Europe samples	Freq from validation data of Asian samples	Freq from validation data of Africa samples	P-value for African vsnon-African
micSeq11220	0.46	0.54	0.63	0.33
micSeq13298	0.11	0.08	0.29	0.043
micSeq16601	0.07	0	0.13	0.31
micSeq18764	0.11	0.08	0.38	0.0021
micSeq11710	0	0.25	0.33	0.0030
micSeq143	0	0.92	0.33	0.61
micSeq1824	0.14	0.29	0.13	0.53
micSeq2373	0	0.08	0.33	0.0010
micSeq2382	0.32	0.42	0.50	0.31
micSeq30	0.25	0.04	0.79	0
micSeq71	0.29	0.29	0.58	0.022
micSeq634	0.14	0	0	0.30
micSeq9196	0.29	0.29	0.92	0
micSeq349	0	0.58	0.75	0.00013
micSeq28	0.54	0.13	0.67	0.013

The sample sizes are 14 for the European group, 12 for the African group, and 12 for the Asian group. For each micSeq, frequency in italic font indicates the maximum frequency. 12 out of 15 micSeqs show higher frequency in the African population than the other populations; 9 of them are statistically significant at the 5% level as indicated by bold font.

ISSN: 1471-2164