Figure 1

Time-course hierarchical clustering and functional annotation of the 3,547 genes that exhibited a significant change in expression levels in the YS during incubation. A. Heatmap showing the expression of the genes before standardization of the data. A blue-red color palette illustrates the log_e gene expression values, with blue corresponding to low and red corresponding to high expression. Clustering of these data is based firstly on the expression levels of the genes and within these levels on expression patterns. B. Heatmap showing the expression of the genes after standardization of the data. A blue-red color palette illustrates the standardized values, with blue corresponding to low and red corresponding to high expression. Cluster analysis was performed on the genes according to their temporal expression profiles and the corresponding dendrogram is shown to the right of the heatmap. The letters on the dendrogram (A, B and C) refer to the 3 major patterns of expression as described in results and discussion. The major 3 clusters were further divided (A1, A2, A3; B1, B2; C1, C2, C3) and colored, as shown to the left of the heatmap. C. DAVID annotation analysis for genes of the 3 major clusters. DAVID enrichment scores were assigned for each functional annotation group.