Figure 5

Expression levels of selected known miRNAs in leaf, flower and fruit. Total RNAs from mixed leaf, mixed flower and fruit in 170DAF were extracted for RNA gel blot analyses; reverse complement probes were used to detect the indicated miRNAs. U6 RNA or 5.8 s rRNA were used as loading controls. Two different genotypes of orange were used. Orange genotype 1 is a common sweet orange while genotype 2 is a red flesh orange. (a) miR482d-5p.2 was specifically expressed in leaf. (b) miR3954a and miR167b.2 were expressed at higher levels in flower than the other tissues; miR5179 was specifically expressed in flower (c) miR3951 and miR164 were expressed at higher levels in fruit than the other tissues; miR477a-3p was specifically expressed in fruit. L1, leaf of orange genotype 1; L2, leaf of orange genotype 2; F1, flower of orange genotype 1; F2, flower of orange genotype 2; Fr1, fruit of orange genotype 1; Fr2, fruit of orange genotype 2.