Expression levels of selected novel miRNAs in leaf, flower and fruit. Total RNAs from mixed leaf, mixed flower and fruit in 170DAF were extracted for RNA gel blot analysis. Reverse complement probes were used to detect specific miRNAs. U6 RNA was used as a loading control for each membrane. Two different genotypes of orange were used. Orange genotype 1 is a common sweet orange while genotype 2 is a red flesh orange. (a) miRN03 and miRN36 were expressed at higher levels in leaf than flower or fruit. (b) miRN08 was specifically expressed in flower. (c) miRN31 was specifically expressed in fruit. L1, leaf of orange genotype 1; L2, leaf of orange genotype 2; F1, flower of orange genotype 1; F2, flower of orange genotype 2; Fr1, fruit of orange genotype 1; Fr2, fruit of orange genotype 2.