Toxicity evaluation of manufactured CeO2 nanoparticles before and after alteration: combined physicochemical and whole-genome expression analysis in Caco-2 cells

Table 3 Microarray gene expression validation

Gene ID	Primers (5′-3′)	Microarray Fold change CeO₂/Ctrl	qRT-PCR Fold Change CeO₂/Ctrl
ATP5J	F:GTCAGCCGTCTCAGTCCATT	−1.94	−1.75
ATP5J	R:AAAAGCTCCCTCTCCAGCTC	−1.94	−1.75
NDUFA4	F:TCCAGATGTTTGTTGGGACA	−2.31	−5.52
NDUFA4	R:GTGGAAAATTGTGCGGATGT	−2.31	−5.52
NDUFS7	F:CGCAAGGTCTACGACCAGAT	−1.68	−2.39
NDUFS7	R:TCCCGCTTGATCTTCCTCT	−1.68	−2.39
PRDX5	F:GTGGTGGCCTGTCTGAGTGT	−1.95	−1.74
PRDX5	R:ATGCCATCCTGTACCACCAT	−1.95	−1.74
Gene ID	Primers (5′-3′)	Microarray Fold change NB-DA / Ctrl	qRT-PCR Fold Change NB-DA / Ctrl
PRDX3	F:GTTGTCGCAGTCTCAGTGGA R:GACGCTCAAATGCTTGATGA	−2.65	−2.98
SDHD	F:GTATGCCTCTTTGCCTCTGC R:GAGGCAACCCCATTAACTCA	−1.84	−4.45
COX6A2	F:CTACCAACACCTCCGCATC R:TCGAAGCTTCACACCTTTATTG	−2.03	−2.08
Gene ID	Primers (5′-3′)	Microarray Fold change NB-DL / Ctrl	qRT-PCR Fold Change NB-DL / Ctrl
SDHC	F:TTGAGTGCAGGGGTCTCTCT R:AACCAGGACAACCACTCCAG	−2.26	−16.30
SDHD	F:GTATGCCTCTTTGCCTCTGC R:GAGGCAACCCCATTAACTCA	−2.03	−22.10

ISSN: 1471-2164