Establishment and analysis of a reference transcriptome for Spodoptera frugiperda

BMC Genomics

Table 1 Biological samples from which RNA has been extracted for the construction of the reference transcriptome

454 RNA sequencing		Illumina RNA sequencing
A. 14 Developmental time-points	B. 13 Dissected tissues	C. 10 samples sequenced by Illumina	D. Abbreviations of the libraries
Eggs	Male adults antennae	Developping eggs	Eggs
Developing eggs*	Female adults antennae	L2 larvae (early stage)	L2e
L1 larvae	Larvae antennae	L2 larvae (late stage)	L2l
L2 larvae (early stage)*	Larvae palps	L3 larvae (early stage)	L3e
L2 larvae (mid-stage)	Adult proboscis	L3 larvae (late stage)	L3l
L2 larvae (late stage)*	Adult brains	L6 larvae (late stage)	L6l
L3 larvae (early stage)*	Larvae heads	Dimboa treated midguts§	MD
L3 larvae (mid-stage)	Hemocytes and imaginal discs	L4 and L5 larvae antennae and palps§	AP
L3 larvae (late stage)*	Salivary glands	Induced fat body§	Fbi
L6 larvae (early stage)	Gonads from female pupae	L5 larvae tracheae§	TrL6
L6 larvae (mid-stage)	Gonads from male pupae
L6 larvae (late stage)*	L5&L6 larvae tracheae
Male pupae	Gut stem cells
Female pupae

This Table shows the description of the samples from which RNA has been extracted. Columns A and B show samples sequenced by 454. They consist of 14 whole organisms developmental time-points (clumn A) and 13 dissected tissues (column B). Column C shows 10 samples sequenced by Illumina and column D shows the abbreviation of the Illumina libraries names that are used in the figures of the paper.
*Those six samples in column A, corresponding to whole organisms developmental time-points, have also been sequenced by Illumina (Column C).
§Those four samples in column C, correspond to dissected tissues that have been sequenced by Illumina only and that were not included in the pooled sample for 454 sequencing.

ISSN: 1471-2164