Differential expression of miRNAs and predicted target mRNAs in G. mellonella pupae and pre-pupae. The miRNAs identified by microarray analysis and their predicted mRNA targets were validated by RT-PCR in order to confirm differential expression in pupae and pre-pupae. They include (A) ame-miR-71 and (B) contig 15133; (C) api-miR-263a and (D) contig 21732; (E) ame-miR-263b and (F) contig 20004. The relative fold changes indicated for the miRNAs and mRNAs have been normalized to aae-miR-252 and 18S rRNA as the internal reference control. (*p < 0.05, ***p < 0.0005, ns = not significant).