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Figure 1 | BMC Genomics

Figure 1

From: Genome wide SNP identification in chickpea for use in development of a high density genetic map and improvement of chickpea reference genome assembly

Figure 1

Schematic diagram describing the experimental approach to develop a high density genetic map of chickpea. A SNP panel of eight chickpea accessions representing desi, kabuli and wild species were used for SNP discovery. 3’-anchored cDNA was sequenced using the Roche 454 Titanium sequencing. SNP calling was done using in-house developed pipeline. Illumina 1536 SNP genotyping assays were developed. CPR-01 was genotyped using Illumina GoldenGate and Restriction site associated DNA (RAD) markers. An integrated high density genetic map was generated from the two data sets. A comparative study between CPR-01 and the recently released draft chickpea genome sequence (Varshney et al. 2013) was conducted. See main text for details.

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BMC Genomics

ISSN: 1471-2164

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