The utility of PacBio circular consensus sequencing for characterizing complex gene families in non-model organisms

Table 3 Polymorphism statistics of DNA sequence data for the V1R subfamilies examined herein

M. murinus1	N	Fragment size (bp)	S	k	π	θ_W	Syn	NonSyn
V1RI (CCS)	29	678	223	55.75	0.083	0.084	160.78	508.22
V1RI (genome)	30	678	225	57.60	0.086	0.084	160.17	508.83
V1RIX (CCS)	24	750	199	42.22	0.056	0.071	186.69	560.31
V1RIX (genome)	19	750	181	42.88	0.057	0.069	186.75	563.25
*M. murinus* 2
V1RI (CCS)	27	678	228	54.83	0.082	0.088	160.95	508.05
V1RI (Sanger)	43	678	271	56.96	0.085	0.093	161.01	507.99
V1RIX (CCS)	30	750	214	45.69	0.061	0.072	187.28	562.72

Number of sequences per methodology (N), number of segregating sites (S), average number of nucleotide differences between sequences (k), mean nucleotide diversity (π), Watterson’s estimator of mutation rate (θ_W), raw number of synonymous (Syn) and nonsynonomous (NonSyn) mutations. CCS = PacBio Circular Consensus Sequencing; genome = sequences mined from the M. murinus draft genome [18]; Sanger = sequences originating from Yoder et al. [29].

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