Characterisation of cultured human glomerular microvascular endothelial cells (HGMEC). A) Phase contrast micrograph of passage 3 purified HGMEC (magnification 200x), B and C) Immunofluorescence images of HGMEC probed for von Willebrand factor and PECAM1, respectively. D) and E) E selectin: HGMEC in panel D) were incubated for 12 hours with TNF alpha prior to fixation. No staining with E selectin was observed in unstimulated cells (E). Texas red conjugated secondary antibodies were used for detection and nuclei were counter stained with Hoechst dye (blue). Original magnification in B-E was 630x.