Figure 5

Relative expression levels of secondary metabolism related gene transcripts in A. racemosus root and leaf tissues after 3, 5 and 12 hours of MeJA treatment. (a) and (b) Represents the q-RT-PCR analysis of glucosyltransferase (GT) and glycosyltransferase (GTF) enzymes identified in transcriptome analysis (c), (d) and (e) represents the expression of cytochrome-p-450 enzymes identified after transcriptome assembly (f), (g), (h), (i) represents the q-RT PCR analysis of other saponin related gene transcripts identified. Total RNA was extracted from root and leaf of one month old plant and expression level of identified transcript was analyzed by q-RT PCR. Actin was used as the internal reference gene and the relative abundance of each gene transcript in root and leaf tissue was compared. The data represents three independent biological and experimental replicates performed with standard deviations.