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Table 3 Comparison of the expression profiles of 26 genes by RNA-seq and quantitative real-time RT-PCR

From: Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

Gene ID Annotation M/CK (log2fold) Z/CK (log2fold) MZ/CK (log2fold)
   RNA-seq qRT-PCR RNA-seq qRT-PCR RNA-seq qRT-PCR
Unigene 1724_All ethylene receptor homolog2 1.72 1.59 ± 0.24 1.19 0.73 ± 0.09 1.87 1.51 ± 0.05
Unigene 1869_All LysM domain receptor-like kinase 3-like 4.26 4.29 ± 0.06 0.51 0.98 ± 0.19 3.97 3.85 ± 0.03
Unigene 2625_All calmodulin binding protein, putative 1.19 0.95 ± 0.16 0.87 0.38 ± 0.15 1.30 0.64 ± 0.10
Unigene 4130_All sugar transporter ERD6-like 7-like 1.74 1.70 ± 0.21 0.84 1.01 ± 0.05 2.03 1.63 ± 0.38
Unigene 4239_All boron transporter 2 0.96 0.58 ± 0.25 -2.17 -3.07 ± 0.06 0.61 0.27 ± 0.39
Unigene 6086_All inorganic phosphate transporter, putative 3.01 2.81 ± 0.34 2.49 2.90 ± 0.26 3.40 3.06 ± 0.10
Unigene 8895_All Xyloglucan endotransglucosylase/hydrolase 2.03 0.58 ± 0.66 2.55 1.74 ± 0.49 1.89 -0.07 ± 0.38
Unigene 10786_All equilibrative nucleoside transporter 3.95 4.71 ± 0.11 -0.21 0.42 ± 0.08 3.94 4.41 ± 0.05
Unigene 12197_All ethylene-responsive element binding factor 4 2.33 1.83 ± 0.53 3.12 2.72 ± 0.39 3.37 2.74 ± 0.11
Unigene 13078_All glutathione peroxidase 4.20 2.62 ± 0.15 1.67 1.04 ± 0.27 3.95 2.45 ± 0.06
Unigene 13219_All transmembrane copper transporter -6.93 -1.01 ± 0.03 8.05 8.15 ± 0.44 6.51 4.65 ± 0.06
Unigene 13639_All ATPase 7, plasma membrane-type-like 4.24 4.39 ± 0.09 0.52 0.32 ± 0.11 3.94 3.81 ± 0.09
Unigene 14415_All major facilitator family protein -2.13 -1.55 ± 0.50 -0.97 -1.13 ± 0.86 -2.36 -1.91 ± 0.50
Unigene 15955_All sorbitol transporter 2.32 2.86 ± 0.38 0.87 1.54 ± 0.21 2.27 2.20 ± 0.07
Unigene 13573_All cellulose synthase A catalytic subunit 4 [UDP-forming]-like 4.76 7.26 ± 0.91 4.94 7.61 ± 0.87 4.67 6.50 ± 0.89
Unigene 2569_All transcription factor, putative 2.18 2.68 ± 0.30 0.00 0.38 ± 0.20 2.00 2.41 ± 0.32
Unigene 14999_All NAC domain-containing protein 43-like 4.19 3.73 ± 0.36 4.60 4.53 ± 0.32 4.32 3.09 ± 0.18
Unigene 16297_All CBL-interacting serine/threonine-protein kinase 11 1.80 1.34 ± 0.35 1.61 1.80 ± 0.18 2.17 1.55 ± 0.35
Unigene 10727_All zinc finger CCCH domain-containing protein 66-like 2.22 2.07 ± 0.24 3.09 2.82 ± 0.04 3.53 2.87 ± 0.01
Unigene 15958_All chitinase 4-like 20.38 13.75 ± 0.15 0.00 -0.04 ± 0.34 20.09 13.40 ± 0.21
Unigene 4429_All laccase-4-like 2.35 2.35 ± 0.29 2.81 3.38 ± 0.06 1.96 1.81 ± 0.19
Unigene 11039_All auxin efflux facilitator PIN1 3.42 3.27 ± 0.39 3.74 2.84 ± 0.07 3.77 3.00 ± 0.25
Unigene 15779_All Xylem serine proteinase 1 precursor, putative 1.54 2.09 ± 0.30 2.40 3.21 ± 0.13 1.79 2.32 ± 0.20
Unigene 2376_All peptide transporter PTR3-A-like 2.92 2.38 ± 0.37 -1.01 -1.44 ± 0.45 2.79 1.70 ± 0.14
Unigene 2386_All oligopeptide transporter 1-like 4.51 4.90 ± 0.49 1.66 2.51 ± 0.46 4.57 4.86 ± 0.49
Unigene 13170_All gibberellin 20-oxidase 1.66 2.13 ± 0.05 0.03 0.45 ± 0.16 1.14 1.00 ± 0.27
  1. Fold changes of selected C. hybridum trasncripts (±standard deviation) were logarithmic transformed which were determined by RNA-seq and qRT-PCR in root tissues treated with isolates ML01 (M) , ZH3A-3 (Z) and ML01+ ZH3A-3 (MZ) at 15 dpi as compared to mock-inoculated control (CK).