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Figure 1 | BMC Genomics

Figure 1

From: Fragmented mitochondrial genomes are present in both major clades of the blood-sucking lice (suborder Anoplura): evidence from two Hoplopleura rodent lice (family Hoplopleuridae)

Figure 1

PCR amplification of the mitochondrial (mt) minichromosomes of the Hoplopleura rodent lice. (A) Lane 1: GeneRuler®100 bp DNA Ladder (Thermo Scientific). Lane 3: PCR amplicons generated with primer pair 249F-249R that spans the coding region of each mt minichromosome of Hoplopleura akanezumi. Lane 5: PCR amplicons generated with primer pair 344F-344R that spans the coding region of each mt minichromosome of Hoplopleura kitti. (B) PCR verification of the mt minichromosomes of Ho. akanezumi. Lane 1, 10 and 14: 500 bp DNA Ladder (TIANGEN). Lane 2-13: PCR amplicons from the 10 minichromosomes and a chimeric mt minichromosome of Ho. akanezumi: atp8-atp6- trnN, trnE-cob- trnS 1 -trnS 2 , trnI- cox1, trnD-trnY 1 - cox2, trnR-nad4L-trnP- cox3 -trnA-trnT, nad2, trnK- nad4, trnC- nad6 -trnW-trnL 2 (taa), rrnS, trnY 2 - rrnL -trnV, chimeric trnY 2 - prrnL - prrnS. (C) PCR verification of the mt minichromosomes of Ho. kitti. Lane 1, 10 and 13: 500 bp DNA Ladder (TIANGEN). Lane 2-13: PCR amplicons from the 11 minichromosomes of Ho. kitti: atp8-atp6- trnN, trnE -cob-trnS 1 -trnS 2 , trnI - cox1, trnD-trnY- cox2 -trnT, trnR-nad4L-trnP- cox3 -trnA, trnQ- nad1 -trnG-nad3, nad2, trnK -nad4, trnC- nad6 -trnW-trnL 2 (taa), rrnS, trnM-trnL 1 (tag)- rrnL -trnV. Genes from which PCR primers were designed are in bold.

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BMC Genomics

ISSN: 1471-2164

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