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Table 2 Quantitative real-time RT-PCR (qRT-PCR) results of 10 selected genes between 4- and 8-cell stage embryos

From: Transcriptional profiles of bovine in vivo pre-implantation development

Comparison

Gene symbol

Log (fold change) RNA-Seq

Expression

Log (fold change)* qRT-PCR

4- vs. 8-cell embryos

GATA6

8.4

Up

10.6

GNB2L1

8.2

Up

9.2

BAD

6.4

Up

8.4

H2AFZ

8.2

Up

10.3

NANOG

11.5

Up

7.8

GDP9

-3.8

Down

-2.1

DNMT1

-3.2

Down

-2.6

ZP2

-3.5

Down

-4.4

STAT3

-2.8

Down

-2.3

 

OOER

-2.7

Down

-2.0

  1. *Fold change is expressed as the ratios of the values of the 4-cell embryos (n = 3) divided by those of the 8-cell embryos (n = 3). Real time RT-PCR results substantiated the differential gene expression patterns from RNA-seq.