Figure 5
From: Transcriptome of Saccharomyces cerevisiae during production of D-xylonate
Comparison of qPCR and microarray analyses of selected genes. Expression profiles of translation-related genes (RPP1B and RPS0B, top panel), genes controlled by the cell wall integrity pathway (PST1 and CCW14; middle panel), and acid stress-associated genes (TPO2 and PMA1, lower panel). The comparison of the two methods shows strong similarity of the profiles from corresponding conditions. The apparently different patterns for the RPP1B, RPS0B, and PMA1 genes are caused by a strong upregulation of these genes in presence of D-glucose, which is captured in the qPCR “0 h” time-point, but not in the microarray “0 h” time-point. Recalculated profiles, considering only the conditions without D-glucose, show significant similarity for the profiles of these genes also (Additional file 8). The shown values represent the average of two (qPCR) and three (microarray) independent cultivations.