Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

Table 4 Differential expression between genders of miRNA and their gene targets

Gene	miRNA¹		Overexpressed in²
			Male						Female
Probe³	Gene symbol⁴	Down-regulated in	miR-218	oni-miR-E218	oni-miR-E224	miR-21	pma-miR-4585	oni-miR-E622	bmo-miR-2779	oni-miR-E255	oni-miR-E304
42507	fcgrt	Female			−	−		−
86	amh		−		−			−			+
41041	urgcp			−	−	−		−
38100	ctnnd2b		−		−	−
25051	g3s1a18
25051	g3s7a7				−
21001	prkcb
41366	zbed3		−						+
41366	7813		−		−
41366	5031		−	−
41052	urgcp		−		−	−		−		+
21060	cyp4f3		−	−	−			−
14085	wasp1					−
22935	urgcp				−	−		−			+
27670	zebd3		−						+
25225	cdh1
39928	gpr144				−	−		−		+	+
16862	ror1		−					−
28545	fam131c	Male	+							−
10109	eci1			+		+
5193	itgb3a					+
13137	timm50		+	+	+	+
555	fndc7					+		+
35393	nkain1							+
6825	kiaa1468				+
7935	rasgef1b		+			+		+
452	higd1a				+	+		+
5723	sult3							+
30073	casp8						+				−
23873	orni-dba							+
20878	hspa5				+
31928	socs7							+
9238	atp5g3				+		+
3491	LOC100703324		+		+
35899	LOC100707490		+				+
800	mrpl34					+		+
23235	psmb8						+
13679	ankrd22					+		+		−
24221	LOC100690239							+
5738	sult3st1							+
25471	rtn4ip1						+
1524	zp3							+
9317	gpa33				+			+
1135	cr/20β-hsd		+				+
Rate of concordant target sites			6/15	2/5	7/17	8/15	6/6^*	13/21	2/2	2/4	3/4

Concordant and discordant differential expression is displayed for nine miRNAs with their forty four differentially expressed between genders predicted gene targets by a false discovery rate of p < 0.05.
¹miR: expressed 3p/5p mature sequence from miRNA precursors.
²Target recognition sites for miRNAs are represented by concordant (+) or discordant (−) differential expression, respectively, assuming that up-regulated miRNAs down-regulate their target sites [74].
³Probe numbers begin with the prefix “CUST_”.
⁴Gene symbol based on NCBI, ENSEMBL or tilapia LOC no. urgcp and zbed3 genes represent different probes at multiple locations in the genome.
^*The probability that the rate of concordant target sites should occur by chance is: (1/2)⁶ = 0.015.

ISSN: 1471-2164