Figure 5
From: Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins
Alignment Domain analysis and phylogenetic tree of Argonaute family of proteins. (A) Amino acid alignment of Argonaute-1 protein of Bombyx mori, Tribolium castaneum, Drosophila melanogaster and Sf21. Indicated sequences were aligned using CLUSTALX. The conserved amino acid residues Asp, Asp and His (DDH) triad residues are marked with red. (B) Domain architecture of Argonaute proteins. The similarity in the domain architectures of Bm-Ago-1 and Sf21-Ago-1 suggests that they are orthologous and the organisation of PAZ/Piwi domain is highly conserved within the insects. (C) The phylogenetic tree was based on consideration of two different class of Argonaute proteins, miRNA class (Argonaute-1) and siRNA class (Argonaute-2). Sf21 genome contains Ago-1 while Tribolium, Bombyx and Drosophila have both Ago-1 and Ago-2.