Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins

BMC Genomics

Table 2 Comparative analysis of Sf21 RNAi genes

Gene (Spodoptera frugiperda) (Lepidoptera)	Bombyx mori(Lepidoptera)		Tribolium castaneum(Coleoptera)		Drosophila melanogaster(Diptera)		Nilaparvata lugens(Hemiptera)		Caenorhabditis elegans(Nematoda)
	A	B	A	B	A	B	A	B	A	B
Argonaute-1	+	-	+	+	+	+	+	+	+	+
Argonaute-3	+	+	+	+	+	+	+	+	Homolog prg-1	+
Dicer-2	+	-	+	+	+	+	+	+	-	-
Dicer-1	-	-	+	+	+	+	+	+	+	+
Aubergine	+	-	+	-	+	+	+	+	Homolog prg-1	+
Drosha	-	-	+	+	+	+	+	₊	+
Pasha	+	-	+	+	+	+	+	+	+	+
Loquacious	+	-	+	+	+	+	+	+	-	-
R2D2	+	-	+	+	+	+	+	+
Dbp45A subfamily	+	-	-	-	+	-	-	-	-	-
VASA subfamily	+	+	+	-	+	+	-	-	-	-
DDX18/HAS1 subfamily	+	-	-	-	+	-	-	-	+	-
U1A snRNP	-	-	+	-	+	-	-	-	+	-
SmG	+	-	+	-	+	-	-	-	+
Integrator complex subunit (Int11)	+	-	-	-	-	-	-	-	+	-
Zn finger protein	+	-	+		+	+	-	-	+	+
Regulator of nonsense transcripts 1 homolog (smg-2 like)	+	-	+	-	+	+	-	-	+	+
CaM Kinase	+	-	+	-	+	-	-	-	+	-
Serine/threonine p21-activated kinase (PAK) mbt like protein	+	-	+	-	+	-	-	-	+	-
cAMP-dependent protein kinase C1	+	-	+	-	+	-	-	-	+	-
Protein Kinase C	+	-	+	-	+	-	-	-	+	-
IKK-beta	+	-	+	-	+	-	-	-	+	-
STE20/Fray	-	-	+	-	+	-	-	-	-	-
MAPKK4	+	-	+	-	+	-	-	-	+	+
MDR1A	+	-	+	-	+	-	-	-	+	+
Tudor	+	+	+	-	+	+	-	-	+	+
Sil-2	+	+	+	+	-	-	+	+	+	+
Histone3 Lysine4 N-methyltransferase	-	-	-	-	+	+	-	-	+	+
Histone deacetylase 3 like	+	-	+	-	+	+	-	-	+	+
Gas41	+	-	+	-	+	-	-	-	-	-
eIF2B-gamma	+	-	+	-	+	-	-	-	+	+
eIF4AII	-	-	-	-	+	-	-	-	Homolog INF-1	-
eIF4AIII	+	-	+	-	+	-	-	-	+	-
RPL23A	+	-	+	-	+	-	-	-	+	+
KIF18A-like	+	-	+	-	-	-	-	-	+	-
Cyclin-dependent kinase 5 homolog	+	-	+	-	+	-	-	-	-	-
KIF3A-like	+	-	+	-	+	-	-	-	+	-
Isocitrate dehydrogenase	+	-	+	-	+	-	-	-	+	+
Myosin VIIa-like	+	-	+	-	+	-	-	-	+	-
Nucleolar complex protein 2 homolog	+	-	+	-	+	-	-	-	+	-
WD 40 like repeat domain	+	-	+	-	+	-	-	-	+	-
S-phase kinase-associated protein (SkpA)	+	-	+	-	+	-	-	-	+	-

The table shows a comparative analysis of identification and validation of candidate genes found in Sf21 with those organisms for which genome wide approach has been performed in search for RNAi factors either by means of computational approach or functional genomics where (A) denotes in sillico identification of the putative candidate and (B) in vivo validation of the same as RNAi factor.

ISSN: 1471-2164