Single molecule sequencing and genome assembly of a clinical specimen of Loa loa,the causative agent of loiasis

BMC Genomics

Table 2 Assembly statistics for contigs

Assembler	Sequencing data	No. contigs	Total length	No. contigs (≥1 kbp)	Total length (≥1 kbp)	Largest contig	GC (%)	N50
CA	Illumina MiSeq	3,785	86,045,090	3,670	85,991,832	362,075	30.7	49,810
MaSurCA	Illumina MiSeq	27,180	96,959,781	16,645	90,159,179	129,528	30.8	10,719
CLCBio	Illumina MiSeq	72,775	104,010,594	12,719	78,215,812	115,234	29.3	7,816
CA	Illumina-corrected PacBio reads	4,436	94,016,717	4,436	94,016,717	901,957	31.0	61,260
CA	Illumina- and 454-corrected PacBio reads	4,222	100,293,095	4,222	100,293,095	905,793	30.8	89,633
HGAP2	PacBio reads	2,601	102,405,157	2,601	102,405,157	1,570,895	30.7	163,655
HGAP2 with trimming	PacBio reads	2,250	96,410,008	2,183	96,369,305	1,570,872	30.8	180,288

This table provides the statistics about the contigs generated by various combinations of assemblers and data sets. The statistics reported include the number of contigs, total length of sequence, number of contigs ≥ 1 kbp, the total length of contigs ≥ 1 kbp, the size of the largest contig, the percent GC, and the N50.

ISSN: 1471-2164