Figure 1

Early and late replication origins show different nucleosomal organization patterns. (A), Heatmap of nucleosome occupancy from 317 replication origins. ARSs are aligned on the Y axis, and the distance from the ACS is indicated on the X axis. Nucleosome occupancy is indicated in orange and nucleosome depletion is indicated in blue. Average nucleosome profiles for the six groups from the k-means clustering are plotted on the right (blue line). (B), Box plot analysis of the NDR width for origin classes shown in A. Classes 3 and 4 were excluded due to their reduced sample size. The bottom and top of the box are the first and third quartiles, and the red band inside the box is the second quartile (the median). The ends of the whiskers represent the lowest and highest data still within 1.5 IQR of the lower and upper quartiles respectively. (C), Comparative nucleosomal profiles of early (blue line) and late (green line) origin groups. (D), Percentage of early (blue) and late (green) replication origins included in the classes established in A. (E), Comparative nucleosomal profiles of class 1-early origins (blue line) and class 6-late origins (green line). (F), Percentage of early (blue) and late (green) replication origins located in intergenic regions between tandem, divergent and convergent transcripts.