Figure 6

The NDR is modified along S phase. (A), ARS603 is represented by a red rectangle and flanking genes, YFL034W and YFL033C, by blue ones. Vertical bars indicate sites for digestion with restriction enzymes PvuII and HindIII. Green rectangle represents the end-terminal probe. The nucleosomal profile from the G1 sample is presented below. (B), Naked DNA and chromatin from wild-type cells collected at 20, 40 60 and 80 min after α-factor arrest and release into YPD were digested with increasing amounts of MNase (indicated with triangles) and subsequently digested with PvuII and HindIII. Samples were electrophoresed, transferred onto a membrane and hybridized. A ladder of polynucleosomes is observed with a prominent region of hypersensitivity to MNase corresponding to the NDR (indicated in brackets in A, B). The black arrow and asterisk point to the changes in chromatin organization observed in the NDR at later time points.