Validation of differentially methylated genes by bisulfite sequencing and differentially expressed genes by Q-PCR. (A) and (B) are the validation results of SCRT1 and SMARCA2 by bisulfate sequencing, respectively. The solid circles represent the methylated CpG locus and the hollow circles represent the unmethylated CpG locus. The Chi-square test showed that there are significant differences between normal and abnormal groups for each of two genes. (C) is the Q-PCR results of five genes. The vertical axis denotes fold change of the RNA-seq and Q-PCR in the abnormal cloned piglets compared to the normal cloned piglets. ALB: albumin; ATF3: activating transcription factor 3; IL6ST: interleukin-6 receptor subunit beta; PDK4: pyruvate dehydrogenase kinase; isozyme 4; CARP: cardiac ankyrin repeat protein.