Figure 9

Validation of the microarray data by qPCR. Irradiated and non-irradiated human PBMCs were cultured for 2, 4, or 20 hours prior to RNA isolation. Expression values were normalized relative to values in non-irradiated PBMCs. Relative gene expression levels were calculated based on the mean value from four samples using the comparative Ct method. Samples were normalized to the housekeeping gene B2M. Data are presented as mean + SD. Genes in panels A-I were up-regulated on the microarray, whereas genes in panels J-N were down-regulated in irradiated samples compared to non-irradiated samples. The qPCR data generally supported the microarray data. *p < 0.05, paired T-test; n = 4.