Figure 4

The BR gene loci of Ch. tentans . The sequence organization of the BR1, BR2, BR3 and BR6 gene loci are shown schematically. Solid blue lines show scaffolds (indicated by capital letters). Previously cloned gene fragments are shown in green. Predicted genes are shown by dark blue arrows and labelled by small letters (direction of the genes are indicated by the arrowheads). Blast hits are shown in pink. In the BR3 locus, the blast hits corresponding to the predicted genes were; gene e: protein FBpp0086723, gene f: protein FBpp0289635, gene g: protein FBpp0075699, gene h: protein FBpp0084614. The positions of the BR genes, in relation to the scaffolds, are shown in yellow in the upper parts of the the images. Each gene (except BR3) is interrupted in the middle. The interruption corresponds to the approximately 35 kb repetitive central part of each gene. Below the BR genes, the black line serves as a length marker. In the BR1 locus, the two purple arrows in opposite direction upstream the BR1 gene represent the palindromic upstream sequences. In the BR2 locus, P1 and P2 (in red) indicate the positions of the probes used for in situ hybridization.