qRT-PCR results of 5 genes relevant to chitin synthesis. The expression patterns of five selected chitin synthesis related genes in the wing disc at six developmental stages. which are 3-day-old fifth instar larvae (L5D3), L5D6, 1-day-old wandering (W1), PP, P0 and 3-day-old pupae (P3). (A) Bm_nscaf3027_063 (hexokinase type 2-like isoform X3). (B) Bm_nscaf2887_059 (glutamine: fructose-6-phosphate aminotransferase). (C) Bm_nscaf2823_132 (glucosamine-6-phosphate N-acetyltransferase). (D) Bm_nscaf2589_266 (chitin synthase A). (E) Bm_nscaf2589_269 (chitin synthase B). The relative expression levels were normalized to the Bmβ-actin levels. The values are the mean ± SEM (n = 3) of three repeat experiments using qRT-PCR. Different letters indicate statistical significance (p < 0.05).