Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis

BMC Genomics

Table 1 Changes in expression levels of the genes involved in the 20E signal transduction pathway

Gene	RNA-Seq ID	L5D6-RPKM	PP-RPKM	P0-RPKM	L5D6 vs PP	PP vs P0
USP ¹	Bm_nscaf2847_250	34.71	16.10	19.17	-	+
	Bm_nscaf2847_251	20.51	14.60	21.78	-	+
ECR A	Bm_nscaf2855_215	0.93	4.57	0.93	+	-
ECR B2	Bm_nscaf2855_217	21.95	2.09	8.55	-3.40*	+
ECR B1	Bm_nscaf2855_219	31.24	24.62	18.58	-	-
E74 ¹	Bm_nscaf2888_430	none	3.07	162.43	+	5.73*
	Bm_nscaf2888_433	19.63	5.34	64.70	-	3.60*
E75B	Bm_nscaf2859_001	45.67	100.29	168.69	-1.13*	+
E75C	Bm_nscaf2859_003	13.21	none	4.83	-	+
HR3	Bm_nscaf2964_066	2.03	268.66	4.91	7.05*	-5.78*
BR-C A-NZ1	Bm_nscaf2970_078	178.90	235.58	67.39	+	-
BR-C Z4	Bm_nscaf2970_079	71.94	122.93	38.45	+	-
β FTZ-F1 ¹	Bm_nscaf1690_227	4.32	9.94	none	+	-13.28*
	Bm_nscaf1690_228	8.48	7.36	1.71	-	-

¹These genes have more than one RNA-Seq sequences. Gene expression level was calculated by RPKM method. "none" means that the expression of the gene was not detected. Asterisks indicate the gene, whose expression level had more than two folds difference between two stages and False Discovery Rate (FDR) was less than 0.001, was identified as differentially expressed at this developmental stage. "+" represents up-regulation, and "-" represents down-regulation. And the values in the last two columns were calculated by Log₂(PP-RPKM/L5D6-RPKM) or Log₂(P0-RPKM/PP-RPKM) and only those differentially expressed genes are given.

ISSN: 1471-2164