Figure 4

Validation of expression of several transcripts by the Nanostring method confirms differential response of some transcripts to auxin in juvenile and mature cuttings. The same RNA samples which were used for the chip analysis were used for the validation. Probes were designed for some selected transcripts and a nanostring detection was performed. Asteriks show statistical significant difference determined by Scheffe analysis as follows: *¹: JA vs JC p < 0.05, *²: JA vs JC p < 0.05, JA vs MC and MA p < 0.01, *³: JA vs MA p < 0.05, JA vs MC p < 0.01, *⁴: JA vs MC and MA p < 0.01, *⁵: JA vs MC and MA p < 0.05, *⁶: JA vs JC and MA p < 0.05, JA vs MC p < 0.01, *⁷: JA vs MC and MA p < 0.01, JA vs JC P < 0.05, *⁸: JA vs JC and MC P < 0.05.