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Figure 1 | BMC Genomics

Figure 1

From: A universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated RNA viruses using the Illumina MiSeq

Figure 1

Read coverage required to obtain an accurate consensus sequence. The consensus sequence resulting from varying levels of coverage was assessed for accuracy. Isolates O/UKG/1450/2001 (blue), O/UKG/1558/2001 (green), O/UKG/1734/2001 (purple), O/UKG/4998/2001 (orange) and O/UKG/14597/2001 (red) alongside the type O exemplar from the serotype panel (black) were analysed. Points on the graph represent a comparison of the identities (scored on the y axis) of a consensus made with total reads and a consensus made with limited read coverage (detailed on the x axis). On average, an identity score of one was maintained up to (and including) a coverage limit of 22 reads. Below this level of coverage, the accuracy of the identities of the compared consensus sequences decreased i.e. consensus sequences made with a depth of 22x reads were identical to the consensus. Sequences created with less than 22x coverage depth were not identical, and therefore considered less accurate.

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BMC Genomics

ISSN: 1471-2164

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