Figure 3

Validation of expression patterns by quantitative PCR (qPCR). Relative gene expression (±SD) in Atlantic halibut embryonic developmental stages estimated by microarray (grey bars; n = 3) and qPCR (white bars; n = 5). The embryonic developmental stages (n = 3) were: 8-cell stage (8CS), germ ring (GR), 10-somite stage (10SS), and hatched larvae (HT). qPCR data were normalized with Luc. Data were standardized against the 8CS stage. A: irf7, B: 90603123, C: HH_90988292, D: eef1a2bp, E: cyp2n, F: HH_193889799, G: HH_90607090, H: HH_166851124, I: HH_90606468, J: rnf213, K: HH_Contig436, L: psmb9, M: HH_90602035, N: HH_90599790, O: pd-1 l, P: ms4a8a, Q: HH_Contig207, and R: mhc1a.