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Table 2 Genes of known function that are differentially regulated in Pro-Pper with respect to amastigotes

From: Stage-specific differential gene expression in Leishmania infantum: from the foregut of Phlebotomus perniciosus to the human phagocyte

Clone

F

Log2R ± S

p

e-value

Def.

Annotation

Annotated gene function

qRT-PCR

    

Fw

Rv

     

Lin13C3

2.72

1.4 ± 0.3

0.011

0

0

b

LinJ.21.0770

Ribonuclease-L inhibitor, ABC subfamily E, putative

 

N.D.

Lin16F1

3.69

1.9 ± 0.4

0.014

-

0

c

LinJ.23.0050

Peroxidoxin/tryparredoxin peroxidase

 

N.D.

Lin16C2

24.97

4.6 ± 0.5

0.004

0

0

b

LinJ.35.3930

EF-hand calmodulin-like protein

+

65.3 ± 3.1

LinJ.35.3940

Hypothetical protein, conserved

 

N.D.

Lin17G12

2.49

1.3 ± 0.4

0.034

0

0

a

LinJ.19.0940

4-coumarate-CoA ligase

 

N.D.

Lin21H10

17.97

4.2 ± 1.0

0.019

0

0

b

LinJ.26.1670

Sphingolipid δ-4 desaturase, putative

+

4.1 ± 0.3

Lin22C9

2.14

1.1 ± 0.4

0.040

0

0

b

LinJ.33.2910

Ubiquitin-conjugating enzyme, putative

 

N.D.

27C6

4.17

2.1 ± 0.2

0.002

0

0

a

LinJ.31.1240

Vacuolar H+-translocating pyrophosphatase, putative

 

N.D.

Lin28C5

7.31

2.9 ± 0.6

0.020

0

0

b

LinJ.26.1670

Sphingolipid δ-4 desaturase, putative

+

4.1 ± 0.3

Lin31D11

3.00

1.6 ± 0.3

0.016

0

0

b

LinJ.31.1870

Protein kinase-like protein

 

N.D.

Lin34F1

2.98

1.6 ± 0.5

0.038

   

LinJ.08.1000

Histone deacetylase, putative

 

N.D.

       

LinJ.26.1620

40S ribosomal protein S33, putative

+

92.2 ± 5.2

LinJ.26.1630

40S ribosomal protein S33, putative

+

92.2 ± 5.2

LinJ.26.1640

Hypothetical protein, conserved

 

N.D.

Lin41C12

2.95

1.6 ± 0.5

0.028

0

0

b

LinJ.31.1600

Cytochrome c oxidase VIII (coxVIII), putative

 

N.D.

Lin45A11

2.35

1.2 ± 0.4

0.033

0

0

b

LinJ.28.2220

Mitochondrial DEAD protein, putative

 

N.D.

Lin48B6

2.60

1.4 ± 0.4

0.026

0

0

b

LinJ.36.2050

Mismatch repair protein MSH8, putative

 

N.D.

Lin49B7

9.21

3.2 ± 1.1

0.039

0

0

b

LinJ.06.1320

Pteridin transporter, putative

 

N.D.

Lin50G5

3.83

1.9 ± 0.4

0.016

0

0

a

LinJ.21.2080

Cytochrome oxidase VI (coxVI), putative

 

N.D.

Lin51A8

2.24

1.2 ± 0.4

0.041

0

0

a

LinJ.32.4190

GIPL-galf transferase, putative

 

N.D.

Lin51E2

2.09

1.1 ± 0.2

0.018

0

0

b

LinJ.36.0020

Histone H4

 

N.D.

Lin51G7

3.85

1.9 ± 0.2

0.003

0

0

b

LinJ.19.1490

Oxidoreductase-like protein

+

3.6 ± 0.2

LinJ.19.1500

Hypothetical protein, conserved

 

N.D.

Lin54C2

7.13

2.8 ± 0.5

0.010

0

0

a

LinJ.06.1310

Mitogen-activated protein kinase

+

10.8 ± 0.5

LinJ.06.1320

Hypothetical protein, conserved

 

N.D.

LinJ.06.1330

Hypothetical protein, conserved

 

N.D.

Lin58H6

5.43

2.4 ± 1.0

0.049

0

0

b

LinJ.08.0030

Vesicle-associated membrane protein, putative

 

N.D.

Lin60H10

4.41

2.1 ± 0.2

0.003

0

0

a

LinJ.23.0630

Oxidoreductase-like protein

+

2.9 ± 0.1

Lin76A1

3.08

1.6 ± 0.4

0.018

0

0

a

LinJ.31.3320

Histone H4

+

11.0 ± 0.4

Lin76F1

5.40

2.4 ± 0.5

0.013

0

0

b

LinJ.34.3370

Phosphatidylinositol 4-kinase, putative

 

N.D.

Lin77B12

2.01

1.0 ± 0.3

0.038

0

0

a

LinJ.27.1520

Eukaryotic translation initiation factor eIF4E, putative

 

N.D.

Lin80B3

2.83

1.5 ± 0.6

0.049

0

0

b

LinJ.28.3250

Glucose-6-phosphate N-acetyltransferase, putative

 

N.D.

Lin82D10

4.25

2.1 ± 0.5

0.019

0

0

a

LinJ.23.0040

β -propeller, putative

+

2.0 ± 0.1

       

LinJ.23.0050

Peroxidoxin/Tryparedoxin peroxidase

+

21.2 ± 0.8

       

LinJ.23.0060

Cyclophilin, putative

 

N.D.

Lin89D8

2.87

1.5 ± 0.4

0.023

0

0

a

LinJ.36.3230

Lipoate protein ligase, putative

+

8.0 ± 0.5

Lin93D6

5.50

2.5 ± 0.3

0.005

0

0

b

LinJ.26.1670/80

Sphingolipid δ-4 desaturase, putative

+

4.1 ± 0.4

       

LinJ.26.1690

Cytochrome c oxidase, subunit V (coxV), putative

 

N.D.

Lin96H7

4.92

2.3 ± 0.3

0.007

0

0

a

LinJ.31.3310

Hypothetical protein, unknown function

 

N.D.

       

LinJ.31.3320

Histone H4, putative

+

11.0 ± 0.4

Lin96B8

4.21

2.1 ± 0.8

0.046

0

0

a

LinJ.31.3310

Hypothetical protein, unknown function

 

N.D.

LinJ.31.3320

Histone H4, putative

+

11.0 ± 0.4

Lin99G6

4.02

2.0 ± 0.5

0.002

4e-156

9e-80

b

LinJ.36.1730

Proteasome subunit β5, putative

+

5.2 ± 0.2

Lin105H8

3.92

2.0 ± 0.6

0.026

0

0

b

LinJ.36.3750

Cysteine synthase, putative

+

3.6 ± 0.0

Lin106G3

2.26

1.2 ± 0.3

0.029

0

0

a

LinJ.31.1070

Biotin/lipoate-protein ligase

+

8.0 ± 0.5

Lin110F5

3.55

1.8 ± 0.3

0.011

0

0

a

LinJ.16.1220

60S ribosomal protein L39, putative

 

N.D.

Lin111D8

9.80

3.3 ± 0.8

0.020

0

0

a

LinJ.08.1000

Histone deacetylase, putative

 

N.D.

Lin113B9

2.72

1.4 ± 0.6

0.048

0

0

a

LinJ.36.0550

Hypothetical protein, conserved

 

N.D.

       

LinJ.36.0560

Protein phosphatase 2C, putative

+

6.4 ± 0.2

       

LinJ.36.0570

Small nuclear ribonucleoprotein, putative

+

9.4 ± 0.8

Lin125F11

5.88

2.5 ± 1.0

0.046

7e-56

3e-55

a

LinJ.32.2780

Cistathionine γ-liase, putative

 

N.D.

Lin130C5

2.52

1.3 ± 0.5

0.040

3e-178

0

b

LinJ.36.3170

Exosome exonuclease RRP41, putative

-

-3.3 ± 0.2

LinJ.36.3180

Clathrin coat assembly protein

 

N.D.

LinJ.36.3190

Pre-mRNA branch site p14 protein, putative

+

43.2 ± 1.3

LinJ.36.3200

Hypothetical protein, conserved

 

N.D.

Lin132A11

5.24

2.4 ± 0.1

0.001

0

0

a

LinJ.31.1240

Vacuolar H+-translocating pyrophosphatase, putative

 

N.D.

Lin136G4

2.66

1.4 ± 0.6

0.049

0

0

b

LinJ.22.1360

Hypothetical protein, conserved

 

N.D.

LinJ.22.1370

60S ribosomal protein L14, putative

+

4.1 ± 0.3

Lin139D8

5.85

2.5 ± 0.6

0.003

0

0

b

LinJ.08.0010

Structural maintenance of chromosome protein 3, putative

 

N.D.

Lin146A12

2.67

1.4 ± 0.4

0.032

0

0

b

LinJ.30.0710

40S ribosomal protein S30, putative

+

56.2 ± 1.7

LinJ.30.0720

NUDC-like protein

 

N.D.

Lin166F2

5.54

2.5 ± 0.7

0.023

0

0

b

LinJ.21.0770

Ribonuclease -L inhibitor, ABC subfamily E, putative

 

N.D.

Lin166H10

2.08

1.1 ± 0.2

0.014

0

0

b

LinJ.26.1680

Sphingolipid δ-4 desaturase, putative

+

4.1 ± 0.4

       

LinJ.26.1690

Cytochrome b5 reductase, putative

 

N.D.

Lin168F2

2.17

1.1 ± 0.2

0.017

0

0

a

LinJ.32.0710

OSM-3-like kinesin

 

N.D.

Lin169E6

6.66

2.7 ± 0.9

0.037

0

0

b

LinJ.32.0550

Profilin, putative

 

N.D.

Lin172B9

4.42

2.1 ± 0.4

0.009

0

0

b

LinJ.26.1680

Sphingolipid δ-4 desaturase, putative

+

4.1 ± 0.4

LinJ.26.1690

Cytochrome b5 reductase, putative

 

N.D.

Lin208F7

3.33

1.7 ± 0.6

0.033

0

0

b

LinJ.30.3640

Ser/Thr protein kinase, putative

 

N.D.

Lin276F6

3.47

1.8 ± 0.3

0.007

0

0

b

LinJ.35.2370

Protein kinase, putative

 

N.D.

Lin290F2

3.52

1.8 ± 0.3

0.012

0

0

b

LinJ.04.1250

Actin

 

N.D.

Lin298H2

7.23

2.8 ± 0.5

0.011

0

0

b

LinJ.22.1340

Ser/Thr protein phosphatase

 

N.D.

Lin299A1

4.34

2.1 ± 0.8

0.045

0

0

b

LinJ.36.1720

Universal minicircle sequence binding protein (UMSBP), putative

 

N.D.

Lin18A12

-2.20

-1.1 ± 0.4

0.044

0

0

b

LinJ.33.2430

UDP-glucose 4′-epimerase

 

N.D.

Lin25B7

-2.34

-1.2 ± 0.4

0.034

0

0

b

LinJ.31.3390

Sodium stibogluconate resistance protein

 

N.D.

Lin30H4

-3.45

-1.8 ± 0.4

0.017

0

2e-111

b

LinJ.27.2500

Glycosomal phosphoenolpyruvate carboxykinase, putative

 

N.D.

Lin35H4

-2.77

-1.5 ± 0.5

0.039

0

0

b

LinJ.34.3740

Expression-site associated glycoprotein (ESAG5), putative

 

N.D.

Lin49D6

-2.83

-1.5 ± 0.5

0.031

0

1e-152

b

LinJ.19.0590

Protein kinase, putative

 

N.D.

Lin54A3

-2.21

-1.1 ± 0.4

0.037

4e-156

0

b

LinJ.36.6510

Small G protein, putative

 

N.D.

Lin77H8

-2.07

-1.0 ± 0.4

0.039

0

0

b

LinJ.08.0690

Amastin-like protein

 

N.D.

Lin88B2

-2.08

-1.1 ± 0.4

0.040

0

0

b

LinJ.10.1070

Histone H3

 

N.D.

Lin101D5

-2.54

-1.3 ± 0.3

0.017

0

2e-28

b

LinJ.27.2500

Glycosomal phosphoenolpyruvate carboxykinase, putative

 

N.D.

Lin101E5

-2.71

-1.4 ± 0.5

0.046

0

0

b

LinJ.35.5330

Protein kinase, putative

 

N.D.

Lin107B10

-2.22

-1.1 ± 0.3

0.003

0

0

b

LinJ.06.1110

Deoxyribose phosphate aldolase, putative

+

-7.3 ± 0.6

       

LinJ.06.1120

Hypothetical protein, conserved

 

N.D.

Lin115H5

-2.37

-1.2 ± 0.4

0.034

0

3e-136

b

LinJ.03.0790

6-phosphofructo-2-kinase/fructose-2,6-diphosphatase, putative

 

N.D.

Lin123E6

-2.11

-1.1 ± 0.3

0.019

0

0

b

LinJ.23.0980

Actin-interacting protein

 

N.D.

Lin188B12

-2.46

-1.3 ± 0.1

0.001

0

0

b

LinJ.31.3400

Sodium stibogluconate-resistance protein

 

N.D.

Lin286D1

-2.41

-1.3 ± 0.4

0.035

0

0

b

LinJ.08.1320

Amastin-like protein

 

N.D.

Lin274G6

-2.25

-1.2 ± 0.4

0.037

0

0

b

LinJ.08.0680/90

Amastin-like protein

 

N.D.

Lin283F3

-2.12

-1.1 ± 0.3

0.023

0

0

b

LinJ.15.0130

ATP-dependent RNA helicase, putative

 

N.D.

Lin283H1

-2.60

-1.4 ± 0.2

0.010

0

0

b

LinJ.21.1670

2-oxoisovalerate dehydrogenase, subunit α, putative

 

N.D.

Lin308D6

-2.11

-1.1 ± 0.1

0.002

0

0

b

LinJ.11.0060

Protein kinase, putative

 

N.D.

  1. Fold changes (up-regulation if F > 2, over the dividing line, and down-regulation if F < -2, below the dividing line), base-two logarithmic scale F and their SD, p, e-values, clone definitions according to mapping outcomes a, b and c, Ids. and annotated functions in the L. infantum genome sequence and the qRT-PCR outcomes are provided. When a given clone overlaps with more than one annotation, only the clones checked for differential regulation using qRT-PCR and non-resolved clones overlapping with resolved clones containing a common gene appear in this table.
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BMC Genomics

ISSN: 1471-2164

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