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Table 1 Summary of the NGS HLA Omixon software sequencing metrics from the MiSeq run

From: High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging

Locus Average depth of all exonsa Allele ratiosb Detection <100% of all exonsc
A 1311 (87.4-2580) 0.7-1.25 38 (96.2-99.9%)
B 1192 (104–2820) 0.78-1.24 112 (92.3-99.9%)
C 1527 (94–3570) 0.59-1.76 114 (98.8-99.9%)
DRB1 475 (26.8-1520) 0.35-3.02 5 (92.9-95.4%)
  1. aAverage exonic sequence read depth per base pair.
  2. bRatio of average sequence read coverage of alleles one and two.
  3. cNumber of alleles with less than 100% sequence coverage.
  4. The range in sequence coverage is indicated in parentheses.