Figure 9
From: Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures
Relative expression of genes associated with podophyllotoxin biosynthesis at 15°C relative to at 25°C. Changes in the abundance of transcripts were analyzed by fragments per kilobase of exon per million fragments mapped (FPKM) as well as quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Name of each gene starts with a prefix Sh that stands for Sinopodophyllum hexandrum. Correlation coefficient of FPKM and qRT-PCR was 0.740 (p-value = 0.009). Actin was used as an internal control and each value represents average of three separate biological replicates. Full name of genes, FPKM data, primers and qRT-PCR condition are given in Additional file 2, Sheet 2.