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Table 4 Comparison between microarray and RT-qPCR data for selected genes

From: Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine: identification of new genes associated with neuronal function and mu opioid receptor expression

Transcripts cluster Id Gene names Microarray data R.fold RT‐qPCR data
Fold change P‐value
Up‐regulation
Dr.24320.1.S1_at Dr.21771.1.S1_at bnip4 2.36/1.23 1.90 ± 0.41* 7.42 E‐05
Dr.9852.1.A1_at camk1γb 2.16 1.90 ± 0.57* 1.41 E‐05
Dr.7717.2.A1_at Dr.7717.1.A1_at grb2 2.02/1.58 1.27 ± 0.15* 8.98 E‐04
Dr.3576.1.A1_at acox1 1.74 2.03 ± 0.43* 2.04 E‐05
Dr.4477.1.A1_at magi1b 1.30 1.55 ± 0.12* 5.43 E‐05
Dr.3645.1.S1_at plg 1.26 −1.06 ± 0.21 0.299
Down‐regulation
Dr.3663.1.A1_at dao.1 −1.94 −3.31 ± 0.11* 3.59 E‐08
Dr.25405.1.A1_at sox19b −1.80 −2.09 ± 0.19* 0.001
Dr.14687.1.A1_at copb2 −1.39 −1.69 ± 0.19* 0.004
Dr.3546.1.S1_at wls −1.25 −1.67 ± 0.16* 0.006
Dr.3211.1.A1_at grik‐l −1.23 −2.94 ± 0.21* 2.53 E‐04
Dr.8118.1.A1_at otpb −1.22 −1.64 ± 0.23* 0.004
  1. Values of fold change by RT-qPCR are given as mean of fold change ± standard deviation. The study includes samples used for microarray hybridization and samples from independent treatments; a total of n = 8. For better comparison of the results obtained with both techniques, microarray and RT-qPCR, Morphine vs. Control fold changes are indicated with positive and negative values for up- and down-regulations, respectively. A one-way t-test was performed to determine whether fold changes obtained for morphine-regulated genes were different from 1 and significant values are indicated with asterisks.