Benzo pyrene-induced DNA adducts and gene expression profiles in target and non-target organs for carcinogenesis in mice

Table 3 Gene expression changes validation with RT-PCR

Symbol	EntrezID	Gene name	LU	SP	FS	LI	CO	GS
P53 related
Trp53	22059	Transformation related protein 53	↓	↓	F	F	F	F
Pcna	18538	Proliferating cell nuclear antigen	↓	↓	-	-	-	-
Ccng1	12450	Cyclin G1	↑	↑	↑	↑	↑	↑
PAH metabolism
Ephx1	13849	Epoxide hydrolase 1, microsomal	↑	↑	-	↑	↑	↑
Cyp1a1	13076	Cytochrome P450, family 1, subfamily a, polypeptide 1	↑	↑	↑	↑	↑	↑
Wnt/ β-catenin signalling
Cdh1	12550	Cadherin 1	-	↑	-	↓	↓	↓
Ctnnb1	12387	catenin	-	-	-	-	↓	↓
Apc	11789	Adenomatosis polyposis coli	-	-	-	-	↓	↓
Stat3 related
Stat 3	20848	Signal transducer and activator of transcription 3	-	↑	↑	↑	↑	↑
Erbb2	13866	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2	↑	F	↓	F	↓	↓
Src	20779	Rous sarcoma oncogene	↑	↑	-	F	-	-
Oncogene and tumour suppressor gene
Fos	14281	FBJ osteosarcoma oncogene	↓	↑	↑	↓	↓	↓
Myc	17869	Myelocytomatosis oncogene	↓	-	-	↑	-	↑
Other genes
Cav	12389	Caveolin, caveolae protein 1	↑	↑	↓	↑	-	↑
Tubb5	22154	Tubulin, beta 5	↓	↓	-	-	-	-
Cdkn2a	12578	Cyclin-dependent kinase inhibitor 2A (P16)	-	↑	↑	↑	F	↑
Ccnd2	12444	Cyclin D2	-	↑	↓	↑	↑	-
Hspa8	15481	Heat shock 70kD protein 8	↑	↑	↑	↑	↑	↑
Hspa5	14828	Heat shock 70kD protein 5	↑	-	↑	-	↑	↑
TNFα	21926	Tumor necrosis factor alpha	↓	-	F	F	↑	F
NFκb	18033	Nuclear factor of kappa light chain gene enhancer in B-cells 1	↓	-	F	F	↓	F

The same RNA sample was measured 3 times independently for each exposure condition, and the consistent changes with a cut-off 1.5 occurring in 3 or more conditions in each organ was considered as being up- or down-regulated. ‘↑’ means up-regulation; ‘↓’ means down-regulation; ‘ – ’ means no change and ‘F’ means no data. LU, lung; SP, spleen, FS, forestomach; LI, liver; CO, colon; GS, glandular stomach.

ISSN: 1471-2164