Figure 3

Immunocytochemistry of protein localization in neuronal dendrites. Fluorescent microscopy evaluation of dendritically localized candidate proteins on cultured rat and mouse cortical neurons hybridized with nine primary antibodies against the protein of interest and detected with Alexa Fluor 546. For each primary antibody images set, the small bottom left corner panels represent MAP2 immuno-staining. Scale bar = 20 μm. (A), Antibodies against SFRS16, ARHGDIA and HNRPK proteins show qualitatively higher dendritic localization in mouse neurons than in rat neurons (Red box). (B), Antibodies against ZFP410, COMMD3 and RSP6 proteins show qualitatively higher dendritic localization in rat neurons than in mouse neurons (Blue box). (C), Antibodies against UBA52, OLFM1 and H2AFZ proteins show dendritic localization in both rat and mouse neurons (Black box).