Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

BMC Genomics

Table 2 Locus-specific biases on the genomic coverage and error rate of the studied WGA systems using low gDNA input (15 cells)

^a: Genomic coverage or call rates is the proportion of target loci giving positive signals over background over the overall number of loci accounted for on Illumina’s Bovine 50 K SNP Chip. ^b: Error rate is the proportion of erroneous genotype calls relatively to the non-amplified reference; All replicates (%): % of loci which consistently covered in all performed replicates; At least 2/3 replicates (%): % of loci which consistently covered in At least 2/3 performed replicates; Any replicate (%): % of loci which consistently covered in any performed replicate; LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

ISSN: 1471-2164