A comprehensive proteomic approach to identifying capacitation related proteins in boar spermatozoa

BMC Genomics

Table 2 Differentially expressed (>3-fold) proteins identified by ESI-MS/MS

Spot no.	gi no.	Symbol	Protein description	Peptide sequence	Mascot score^*
2209	gi\|298160982	ROPN1	Ropporin-1A	R.LIIHADELAQMWK.V	105
				R.VALSNWAELTPELLK.I
				R.MLNYIEQEVIGPDGLIK.V
3515	gi\|346986351	PDHB	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	R.IMEGPAFNFLDAPAVR.V	245
				K.TYYMSGGLQSVPIVFR.G
				K.TTHLITVEGGWPQFGIGAEICAR.I
5206	gi\|464526	RAB2	Ras-related protein Rab-2	R.GAAGALLVYDITR.R	248
				R.DTFNHLTTWLEDAR.Q
				R.FQPVHDLTIGVEFGAR.M
5404	gi\|89153	APOA1	Apolipoprotein A-I	R.DYVAQFEASALGK.H	34
5712	gi\|21263966	SUCLA2	Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial	K.LHGGTPANFLDVGGGATVHQVTEAFK.L	44
6207	gi\|47523086	PRDX5	Peroxiredoxin-5, mitochondrial	R.LLADPTGAFGK.E	150
				R.FSMVIEDGIVK.S
				K.VGDAIPSVVVFEGEPEKK.V
7106	gi\|75052483	ACRBP	Acrosin-binding protein	R.FYGLDLYGGLR.M	125
7106	gi\|75052483	ACRBP	Acrosin-binding protein	R.VASWLQTEFLSFQDGDFPTK.I	125
8326	gi\|13195731	GPX4	Phospholipid hydroperoxide glutathione peroxidase	K.TEVNYTQLVDLHAR.Y	109
8326	gi\|13195731	GPX4	Phospholipid hydroperoxide glutathione peroxidase	R.QEPGSDAEIKEFAAGYNVK.F	109
9117	gi\|66990208	AWN	Spermadhesin AWN	K.EYVELLDGPPGSEIIGK.I	173
9117	gi\|66990208	AWN	Spermadhesin AWN	R.ASPFHIYYYADPEGPLPFPYFER.Q	173
9212	gi\|113205666	LOC733605	NADH dehydrogenase 1 beta subcomplex 6	R.IFPGDTILETGEVIPLMK.E	48

*MASCOT score is -10 log (P), where P is the probability that the observed match is a random event. Individual scores >30 indicate identity or extensive homology (P < 0.05).

ISSN: 1471-2164