Figure 2

Metabolic regulation of Methylacidiphilum fumariolicum SolV cell cultures grown under different conditions. A) Circos plot depicts the genome-wide expression profile for cell cultures under maximum growth conditions (blue) and the relative gene expressions (fold change) of cell cultures grown under nitrogen fixation or oxygen limitation conditions. Count-per-million (CPM) was used to determine the level of gene expression. Key biological pathways are highlighted in different colours. B) MA plot for cell cultures under nitrogen fixation condition as compared to cell cultures in maximum growth environment. Deregulated genes are depicted in red. MA plot for cell cultures under oxygen limitation condition as compared to cell cultures in maximum growth environment. Deregulated genes are depicted in red. C) Venn diagram shows the number of genes that are differentially expression in both N₂fix and O₂lim conditions compared to μmax. Pie charts illustrate the fraction of genes that have a higher (black) or lower (light grey) expression in N₂fix and O₂lim cell cultures relative to μmax. D) Bar charts present the fraction of up- or down-regulated genes (black and light grey, respectively) in each of the nine key pathways. Red line depicts the 50% mark. The proportion of non-significant genes is depicted in white.