Figure 2

MET1 phylogenetic trees in flowering plants and syntenic conservation. A) Maximum likelihood (ML) phylogenetic tree. The ML tree was inferred using cDNA sequences from dicot species including Arabidopsis thaliana (AtMET1: [TAIR:At5g49160]), Brassica rapa (BrMET1a and BrMET1b respectively [GenBank: AB251938 and AB25937]), Pisum sativum (PsMET1: [GenBank: AF034419]), Medicago truncatula: (MtMET1: [Phytozome: Medtr6g065580]), Daucus carota (DcMET1: [GenBank: AF007807]), Nicotiana tabacum (NtMET1: [Genbank: AB030726]), Solanum lycopersicum (SlMET1: [GenBank: AJ002140]), and monocot species including Sorghum bicolor (SbMET1a and SbMET1b respectively [Phytozome: Sb01g005084 and Sb02g004680]), Zea mays (ZmMET1b-1 and ZmMET1b-2 respectively [Phytozome: GRMZM2G333916 and GRMZM2G334041]), Oriza sativa (OsMET1a and OsMET1b respectively: [Genbank AB362510 and AB362511]), Brachypodium distachyon (BdMET1a and BdMET1b respectively [Phytozome: Bradi1g05380 and Bradi1g55287]), Hordeum vulgare (HvMET1-2 and HvMET1-7 respectively: [Ensembl Genomes: MLOC_61904.6 and: MLOC_10988.2], HvMET1-5 predicted from [GenBank: CAJW010043285]) and Triticum aestivum: TaMET-2A1, 2B1, 2D1, 5A1, 5B1, 5D1, 7A1, 7B1 and 7D1 (this study from IWGSC contigs). Numbers above branches indicate bootstrap values. Two monophyletic groups were defined and called the MET-1a and MET-1b lineages according to the OsMET1a and OsMET1b genes (indicated in boxes). The two gene duplication events observed in wheat and barley are indicated by an arrow. B) Micro-synteny analysis. Micro-synteny was established by BLASTn analysis between genes from brachypodium, rice and IWGSC sequences from wheat chromosomes as described in Methods. Syntenic conservation between rice (in white) and brachypodium (in black) correspond to the number of conserved genes in wheat/number of genes in the syntenic region from brachypodium or rice expressed as percentage. C) Gene structures of MET1 genes at chromosome 5. BLASTn analysis was used to align MET1 genes from Hordeum vulgare (HvMET1-5: [GenBank: CAJW010043285]), Triticum urartu (TuMET1-5 [Ensembl Plants: scaffold15783]), Aegilops tauschii (AetMET1-5 [Ensembl Plants: scaffold164515]) and Triticum aestivum (TaMET-5A1 and TaMET-5D1) (this study).