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Figure 2 | BMC Genomics

Figure 2

From: Discovery of genetic biomarkers contributing to variation in drug response of cytidine analogues using human lymphoblastoid cell lines

Figure 2

Schematic diagram of the strategy for candidate gene selection for functional validation. Genome-wide association studies for either gemcitabine or AraC cytotoxicity were performed with 1.3 million SNPs or 54,000 expression probe sets. “Integrated analyses” were performed using SNP “loci” that contained at least 2 SNPs (P < 10-4) or 1 SNP (P < 10-4) plus 3 SNPs (P < 10-3) within 100 kb surrounding the top significant SNPs, 54,000 expression probe sets and IC50 values to identify SNPs associated with drug IC50 values through their influence on gene expression (SNP-Expression P value <10-4, Expression-IC50 P value <10-4). Finally, 26 candidate genes, including 11 for gemcitabine, 10 for AraC, and 5 for both, were selected for functional validation studies that were performed with multiple cancer cell lines.

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BMC Genomics

ISSN: 1471-2164

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