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Table 4 Functional studies of candidate genes

From: Discovery of genetic biomarkers contributing to variation in drug response of cytidine analogues using human lymphoblastoid cell lines

Gene symbol Caspase-3/7 activity Cancer-related Cignal Reporter Array
Wnt (TCF/LEF) Notch (RBP-Jκ) p53/DNA damage (p53) TGFβ (SMAD2/3/4) cell cycle (E2F/DP1) NFκB (NFκB) c-Myc (Myc/Max) Hypoxia (HIF1A) MAPK /ERK (Elk-1/SRF) MAPK /JNK (AP-1)
PIGB Decrease No No No No No Decrease Decrease No Decrease Decrease
ZADH2 Decrease No No No No No No Increase No No No
PSME3 Decrease No Increase No Decrease No No Increase Decrease No No
DOK6 Decrease No No No No No Decrease No No No Decrease
TGFBI Decrease No No Increase No No Decrease Decrease Decrease Decrease Decrease
C14orf169 Decrease No Increase Increase No No Increase Increase No Increase Increase
TUSC3 Decrease No No No Decrease Decrease No No Decrease No No
RIS1 (TMEM158) No No No No No No No Increase No No No
LNX2 No No No No No No No No No Increase No
SMC2 No No No No No No No No No No No
HLA-DRA Decrease No Increase No No No Increase No No No No
  1. Functional characterization of 11 genes using the Cancer-related Cignal Reporter Array (SABioscience).
  2. Transcription factors for each signal pathway are listed in parenthesis. “Increase” means at least a 2-fold increase of luciferase activity in cells treated with specific siRNA compared with cells treated with negative control siRNA; while “decrease” indicates at least a 50% decrease of luciferase activity in control cells. “No” means no alteration in luciferase activity after siRNA knockdown.