Identification and characterization of crude membrane proteome. (A) Overlap between proteins identified from 2 biological sets. The number of unique protein groups and the corresponding gene symbols identified from the 2 biological sets are described. The number of nonredundant genes that were identified from each biological set is shown in parentheses. (B) Percentage of identified proteins by functional category. All identified proteins in the 2 biological replicates were grouped into 6 categories: total identified proteins (Total I.D), proteins annotated as GO term “membrane” (GO:membrane), proteins annotated as GO term “integral to membrane” (GO:integral to membrane), proteins annotated as GO term “plasma membrane” (GO:plasma membrane), transmembrane domain (TMD)-containing proteins, and proteins with GRAVY score above 0 (GRAVY > 0). The total number of proteins identified in each category is indicated on the right of the bar. Blue bars represent percentage of proteins identified in all 2 biological sets. Red and green bars indicate percentage of proteins detected only in biological sets 1 and 2, respectively. (C) Distribution of number of unique proteins versus the number of predicted transmembrane domains. (D) Distribution of grand average hydrophobicity (GRAVY) score and molecular weight (MW) of proteins identified in the 2 biological replicates. Y-axis is the GRAVY score value, and the x-axis represents logarithmic molecular weights of the identified proteins. The GRAVY scores and log MW of 2164 transmembrane domain (TMD)-containing proteins, merged from 2 biological sets, are plotted as edged blue circles.